Caylee Marie Anthony, 2, FL Missing since June 16-just reported by mother #14

[GreatOwl]

Yes.  Brian of Brians Dreams has been working together with Gayle of Body Hunters.  Same one 

Ok, I know the background then.  Thanks.

[KnayKnay]

I changed my avitar for a little while to show off my grandbaby T.J.

Very cute grandbaby you got there, KnayKnay. 

Adorable grandbaby KnayKnay!  They are such a blessing!

Yes and he's my very first!!! So we're really enjoying ahem spoiling him.

[Destiny]

Thanks guys!!!!!

Nite Sweet Pea....Des...

[Minaa]

Thats insane that they need help via a personal assistant to sit around and do the
so called phone call search for Caylee.
 

[Daryl]

That's not correct at all. We use chloroform all the time. Along with being an old fashioned anesthetic chloroform is a standard ingredient in a forensic Phenol/Chloroform DNA extraction method. Just a guess, check the LE boyfriend. Seriously, we have pints of the stuff in the fume hoods.

If it is used for DNA extraction does that mean it is possible for it to have came from the CSI  while they were doing the forensics on the car?

I so want it to be something to help convict Casey but I also would want to know for sure. JMHO

Could very well be from LE activities. Chloroform is extremely volatile and I don't see how it by itself could have survived many hot days in a Florida car trunk. Unless, and this starts to get a little complicated, so unless you are really interested in this you can depart, chloroform complexes with benzene and other benzoate derivatives. These are anti-fungal compounds commonly found in household detergents. How could this happen? There would have had to be detergents left in the trunk, incomplete rinsing, followed by chloroform from LE to allow the complexed chloroform to remain and be detected. Just my considered opinion. (I did a thesis on this.)

Daryl - how would you explain the computer activity?

one more question. Why would they be using chloroform in trunk for DNA testing...wouldn't that be done in the lab? Is that routine to test on location like that?

Ok sorry I HAVE to chime in here.  No offense to opposing Monkeys...BUT...looking back at the photo's taken of the CSI's collecting evidence, they were in full on suites just to insure against cross contamination.. I highly HIGHLY doubt that one of them would have been so brain lost that day to accidentally over pour on the chloroform, IF in fact it was used in the particular collection of evidence.  I do not see the point in using the solvent to collect DNA evidence as it would defeat the whole purpose by washing the evidence away.  IMO  Sorry if I have offended..was not my intention.

You are not offending me, I agree with you. Chloroform is a carcinogen. No one uses it without training. Maybe someone can check and see whether one can buy chloroform using a personal credit card from Sigma or Fisher. I would hope that would not work.

Anyway, there is a standard Water/Phenol/Chloroform/Isoamyl alcohol extraction for DNA. The DNA goes into the water phase. The PCI kills any environmental enzymes that can degrade the DNA. Nothing is washed away.

The problem is DNA is rather sticky. It adheres to sand, glass, lots of nitrocellulose plastics etc. You have to use something to get it off the substrate and into the analysis. This stuff just seems to work. A better question would be to ask Orange County SO if they used PCI to release their DNA samples. I would tend to believe the chloroform is a red herring or indicates car washing followed by LE sample gathering.

[OIC,UR12!]

That's not correct at all. We use chloroform all the time. Along with being an old fashioned anesthetic chloroform is a standard ingredient in a forensic Phenol/Chloroform DNA extraction method. Just a guess, check the LE boyfriend. Seriously, we have pints of the stuff in the fume hoods.

If it is used for DNA extraction does that mean it is possible for it to have came from the CSI  while they were doing the forensics on the car?

I so want it to be something to help convict Casey but I also would want to know for sure. JMHO

Could very well be from LE activities. Chloroform is extremely volatile and I don't see how it by itself could have survived many hot days in a Florida car trunk. Unless, and this starts to get a little complicated, so unless you are really interested in this you can depart, chloroform complexes with benzene and other benzoate derivatives. These are anti-fungal compounds commonly found in household detergents. How could this happen? There would have had to be detergents left in the trunk, incomplete rinsing, followed by chloroform from LE to allow the complexed chloroform to remain and be detected. Just my considered opinion. (I did a thesis on this.)

new here, but I thought some might want to hear my two cents worth, so here goes. The detergent bottles, maybe SOMEBODY tried to wash something out of the trunk of that car, and that SOMEBODY did not get it rinsed out very well leading to the detection of the chloroform... and wonder who would have thought to wash away evidence of a dead body, even evidence the naked eye would not see... Wonder if there is a car wash located near where the car was found, or Tony's apt? could have been a place to find possible evidence if time had not passed by...

just some thoughts...

[klaasend]

Klaasend:

My post 1076, should be removed.  It was my replay to a previous post, and I entered it in the wrong spot, so it looks like another poster said it... I reposted it later to correct it.  Sorry.  TIA

Done.  Sometimes posts get quoted "funky".  BamaMonkey's post with the quote was funky and all other quotes afterwards carried over the funkyness.  Hard to fix somethimes 

[Tamikosmom]

Ok I'm confused.  They said on news that maybe the LE used too much cholorform in the DNA testing and got it in the trunk?????

... and it was just a cooincidence that Casey was googling the internet for information on cholorform.

That's it.  It is all a cooincident ... a huge misunderstanding.

 

Janet

[ldstlou]

Goodnight Lisa 

nite Muffy!!! I am staying waaaay below that last [/quote] thingy!!!
Did I make it?

[MuffyBee]

Thats insane that they need help via a personal assistant to sit around and do the
so called phone call search for Caylee.
 

Didn't they also say the personal assistant would need to be able to travel?  It's one thing to volunteer to make phone calls, but to travel?  How many unpaid personal assistants would do that? 

[ldstlou]

Klaasend:

My post 1076, should be removed.  It was my replay to a previous post, and I entered it in the wrong spot, so it looks like another poster said it... I reposted it later to correct it.  Sorry.  TIA

Done.  Sometimes posts get quoted "funky".  BamaMonkey's post with the quote was funky and all other quotes afterwards carried over the funkyness.  Hard to fix somethimes 

I knew you were punking me Klaas!!!!

 

[MuffyBee]

Goodnight Lisa 

nite Muffy!!! I am staying waaaay below that last

thingy!!!
Did I make it?
[/quote]


Yes Lisa, you did good.  Goodnight to you and your son. 

[Destiny]

Great Show Dana...Thank You for all you do....Destiny

[klaasend]

That's not correct at all. We use chloroform all the time. Along with being an old fashioned anesthetic chloroform is a standard ingredient in a forensic Phenol/Chloroform DNA extraction method. Just a guess, check the LE boyfriend. Seriously, we have pints of the stuff in the fume hoods.

If it is used for DNA extraction does that mean it is possible for it to have came from the CSI  while they were doing the forensics on the car?

I so want it to be something to help convict Casey but I also would want to know for sure. JMHO

Could very well be from LE activities. Chloroform is extremely volatile and I don't see how it by itself could have survived many hot days in a Florida car trunk. Unless, and this starts to get a little complicated, so unless you are really interested in this you can depart, chloroform complexes with benzene and other benzoate derivatives. These are anti-fungal compounds commonly found in household detergents. How could this happen? There would have had to be detergents left in the trunk, incomplete rinsing, followed by chloroform from LE to allow the complexed chloroform to remain and be detected. Just my considered opinion. (I did a thesis on this.)

new here, but I thought some might want to hear my two cents worth, so here goes. The detergent bottles, maybe SOMEBODY tried to wash something out of the trunk of that car, and that SOMEBODY did not get it rinsed out very well leading to the detection of the chloroform... and wonder who would have thought to wash away evidence of a dead body, even evidence the naked eye would not see... Wonder if there is a car wash located near where the car was found, or Tony's apt? could have been a place to find possible evidence if time had not passed by...

just some thoughts...

Welcome OIC!

Great thoughts but I keep going back to the computer searches for chloroform.   Why?

[Sam]

Hi Sam ~  I have one of those kids that is hell on wheels too    I wish we had more information about a lot of things in this case. 

We all want more. Enjoy that Kid Muffee they grow up so fast.

Knay Knay your grandchild is gorgeous.


And to whoever made that original first post about if they were Tony they would have been worried. I agree. Also remember in he dream thingy she said everyone lies everyone dies. Could have been a reverence to her parents as well.

[MuffyBee]

The computer searches for chloroform does sound mighty suspicious. 

[klaasend]

http://www.helpfindcaylee.com/contact

Volunteer Positions Needed
 

Publix Tent/Booth Attendant

8:00am to 6:00pm DAILY  (partial availability welcome!)

Location:  Publix Supermarket located on the corner of Chickasaw Trail and Lake Underhill Road
Purpose:  To provide an established location where members of the community can stop by to obtain fliers and other supplies, donate to the Help Find Caylee Trust Fund, or sign up for other volunteer opportunities.

Whom to Contact:  George Anthony - georgehope4937@yahoo.com
 
Flier Distributor

8:00am to 6:00pm DAILY  (partial availability welcome!)
Meet Locations:  Publix Supermarket located on corner of Chickasaw Trail and Lake Underhill Road or Charity office space located near Sand Lake Road and Orange Blossom Trail.
Purpose:  Visit businesses door to door to post fliers, stand outside "high-traffic" businesses/churches to distribute to the public, put fliers on car windshields, etc.

Whom to Contact:  George Anthony - georgehope4937@yahoo.com
 
Call Center Representative

9:00am to 10:00pm DAILY except Sundays  (partial availability welcome!)
Location:  Charity office space located near Sand Lake Road and Orange Blossom Trail.
Purpose:  Answer phone calls from the public that wish to provide tips or information on Caylee's whereabouts since she went missing on June 16, 2008.

Whom to Contact:  George Anthony - georgehope4937@yahoo.com

[Minaa]

Im nosey, but i am dying to know what was bought at target with the stolen checks.

[klaasend]

The computer searches for chloroform does sound mighty suspicious. 

It will be interresting when we find out the dates of these searches. 

[Rob]

Thats insane that they need help via a personal assistant to sit around and do the
so called phone call search for Caylee.
 

Can you imagine how horrible they would have treated those servants?

they have kicked everyone that has tried to help them square in the whisker biscuit.