Caylee Marie Anthony, 2, FL Missing since June 16-just reported by mother #16

[klaasend]

Klaas? Can you or someone please tell me WTF that stuff was about the house next door being searched?? If there was something to it, then please forgive me..But I am just really wondering because I have found NOTHING on it....

Nikki - we don't know.  What we heard is someone in Florida was saying that on local news it looked like they had search dogs at a house down the street from the Anthony's (not right next door).  That's all we ever heard.

[nikkibnurse]

Klaas? Can you or someone please tell me WTF that stuff was about the house next door being searched?? If there was something to it, then please forgive me..But I am just really wondering because I have found NOTHING on it....

PERSONALLY I think it was possibly someone tryin to stir up the cage .....  They left kind of abruptly when the 5:30 breaking news was pointed out at 6:30

Thats what I was thinkin'..and that pi$$es me off!!

[KarmaRoundUp]

Theory:  Casey did in house or yard.  Buried in yard.  Moved to the car for several days.
Came home for ? reason (get clean clothes, get gas cans, get help from Dad).  Body removed from car and put in freezer.  Can't get smell out of car.  Try cleaning/sanitizing for DNA.  Abandon car.  Smell still there though.

Remember, we heard that LE has over 20 pieces of evidence/results that they have not yet released the information on to the public.



Also,  LP saying on NG that after last Wed. the A's attitude changed.  Well...didn't we already find out that last Wed. Lee, Cindy and Casey were told of the evidence the LE has??  LP said tonight that they "know" what happened to Caylee.

I HIGHLY DOUBT that it was because Casey told Lee and then Lee told Cindy.  That part doesn't make sense to me. 

Could the body still be in the house somewhere? Freezer? Under boards in yard - under the pool?

Wasn't it said that they got new concrete poured on the weekend of the 4th or somewhere around there?

I'm not sure if that was a rumor or not but I DO see a new sidewalk to the left of their garage,by the street.

[SS]

I believe the Leonard Padilla should have stopped and considered his words prior to implying that Lee Anthony could be Casey's father.

Janet.
_________

New Twist In Casey Anthony Case
Thursday, September 4, 2008
Brother may be tied to case
by Rozzie Franco and Nada Taha, 540 WFLA/FNN

Brother may be tied to case
by Rozzie Franco and Nada Taha, 540 WFLA/FNN

ORLANDO, FLA. --   The latest twist in the Casey Anthony case comes from the man who at one point gave her a “get out of jail free” card and may tie her brother, Lee Anthony, into the saga ...

Padilla said that officials ruled it was Caylee’s hair just by a process of elimination because the hair had a death ring around it and they know Casey is alive. The fact that the DNA is identical has something to do with who the father is, Padilla said.

“Would the DNA from a child resulting from a union from Lee and Casey have basically the same characteristics? Making the preliminary DNA check not definitive as to who the hair samples are from,” he said.

http://www.540wfla.com/cc-common/mainheadlines3.html?feed=227698&article=4189759

+++++++

Mitochondrial DNA: Mitochondrial DNA: Within the cell cytoplasm there are organelles called mitochondria. The mictochondria contain mitochondrial DNA (mtDNA) which is the type of DNA analyzed in order to identify deceased remains. Mitochondrial DNA is inherited from the mother, not from the father.called mitochondria. The mictochondria contain mitochondrial DNA (mtDNA) which is the type of DNA analyzed in order to identify deceased remains. Mitochondrial DNA is inherited from the mother, not from the father.

http://travel.state.gov/visa/immigrants/info/info_1337.html

+++++++++++

Quote from: mbs on September 04, 2008, 03:59:12 PM

A mother's mitochondrial DNA is always identical to her children's mitochondrial DNA.  If all they tested was mitochondrial, then they would not be able to distinguish between Casey and Caylee.

http://en.wikipedia.org/wiki/Mitochondrial_DNA

http://scaredmonkeys.net/index.php?topic=3330.msg434990#msg434990

Janet, I agree with what you are saying because I researched the types of DNA testing, also.  Something continues to nag at me though.  This is such a high profile case with so many possible motives and people involved.  Testing was done at The Body Farm in Tennessee and at Quantico.  I would think that LE would have done all types of DNA testing and several times for accuracy.  It did take weeks before the DNA results were leaked.  I feel that they probably did both DNA tests.

SS

It was implied a couple pages back in a post that Mitochondrial DNA testing takes a shorter period of time to determine results.  It could be that further DNA results are forthcoming.  Hey ... I do not have a clue.

Keep in mind ... the purpose of the testing was to determine if Caylee's DNA was present in the trunk of that vehicle.  It only takes Mitochondrial testing to determine that.

However ... I believe that there should be an official confirmation regarding what types of DNA testing was conducted prior to implying that Lee or George Anthony is Caylee's father.  I contend that our speculation should have some boundaries.

Janet

Janet, you're absolutely correct.  They should have qualified the DNA test results.  Unfortunately, I think that there were probably ulterior motives behind releasing the information.  The public was given only limited information, but enough to indicate that there was a likelihood of incest.  Unfortunately, I also think that this was intentional.  I wonder if it was done to create stress with the Anthonys in hopes that one of them would break.

[nikkibnurse]

Thanks Klaas

[Kristin]

5 Ways to Clean Up A DNA Sample
October 25, 2007 | By Nick In Tech Tips |




One of the most common tasks in molecular biology is cleaning up DNA from aqueous solutions to remove buffer salts, enzymes or other substances that could affect downstream applications. Examples include cleaning up PCR reactions, digests or other enzymatic treatments and cleaning up genomic or plasmid DNA contaminated with cellular proteins/debris. There are several ways to approach DNA clean-up, here are five of them.

1. Phenol/Chloroform extraction

Phenol chloroform extraction (see Kirby, 1957), normally followed by ethanol precipitation, is the traditional method to remove protein from a DNA sample. In this procedure, the DNA solution is mixed with phenol and chloroform. The water-soluble DNA partitions into the aqueous phase, while the proteins are denatured by the organic solvents and stay in the organic phase. The aqueous phase containing the protein-free DNA can then be collected.

Advantages: A cheap and effective way to remove proteins from DNA solutions
Disadvantages: Slow compared to most modern methods, there is a risk of phenol/chloroform carry-over into the final sample (which could inhibit downstream enzymatic reactions), chloroform and phenol are both hazardous chemicals

[Lynn32792]

This is Stephanie's (Stephanie Kostakis) Myspace page:

http://profile.myspace.com/index.cfm?fuseaction=user.viewprofile&friendid=29472255

[anothermonkey]

over on Topix:
http://www.topix.net/forum/county/orange-fl/T4UHQV6DMIUFOA9C5/p1450

Duh in Orlando

Sorry about the brief report earlier. I crashed. This is just a kind of picture of what goes on at TES.

The people who came with horses are assigned to a special group. They are sent out to areas that are pretty wide open. There were 6 private airboats, they were sent out to local lakes, not ponds.

The 150 or so walkers are divided up into groups of 10 with one leader who can read maps, has a gps and a cell phone. One or more groups are given a map of an area to search. The area I went to had 3 groups. We were sent to a rural community about 13 miles from HQ. This community has several large tracks of land around it. Some of those tracks are known for kids partying at night. The one we went to had a main trail. The rest of the area was scrub pines, palmettos and vines. We had two dog teams with us. This gets a little rough, so don't read the rest. We were told we were not looking for the little girl on the T shirts. We were looking for small bones that may have been spread out by animals. Walking thru palmetos is an interesting experience. It turned out that the locals in this area must do a lot of poaching here and dumping of carcassas. We found a lot of bones. It rained most of the day and about 30% of the area was under water. The other problem is this time of year the pines shead there 6 inch needles, which has been made even worse by the recent storms. There is a thick layer of them on the ground. One poor lady found a garbage bag of bones. They turned out to be pig ribs. It rained all day but was about 85 degrees. But strangely I felt cold all day. I think maybe only hunters may know the feeling in a way, that during the season, you look around and at a hundred yards every tree branch looks like antlers. Here it was every white leaf, every white moss stick on the ground looks like a bone. All I can say is there were a lot of very nice folks on a treasure hunt that no one wants to win. But, the more people that are out there, the greater chance of finding something. Before today, I wanted to go out. Now I am compelled. If my legs are working tomorrow.....lol...we will give it a shot. Otherwise, Sunday for sure.

If you guys have any questions let me know.

And let me say again. Tim Miller is the real deal. Probably the only real deal we will meet in this story.

[Rob]

If I was Casy, I would almost rather be known as a child murderer than to admit she allowed her child to be sexually abused by child pornographers.

I guess in a woman's prison there are husband and child killers. That's pretty bad - but to be expected in a woman's prison.

A woman that pimped her kid who was killed would be a level or twelve below those folks.

Thats right rob....and women in prison who did not kill their children, are dying to see them..so, they have no respect for someone who kills their kids.....turn her loose in general pop.....she wont last 5 minutes

Yep! Actually we may be going about this all wrong. Convict her ONLY on the petty crimes and put her in the general population! If she went to Maximum security -- she would probably be in solitary.

good thinking wreck

except she won't be facing these charges until November at the earliest... so she will remain out on bond pending new charges.

[KarmaRoundUp]

I can't seem to find any document stating the date LE picked up the Pontiac and took it to their lab.

Can anyone help me on this?

Brandi,I think it was the 17th of June....I could be wrong though.

[invision]

5 Ways to Clean Up A DNA Sample
October 25, 2007 | By Nick In Tech Tips |




One of the most common tasks in molecular biology is cleaning up DNA from aqueous solutions to remove buffer salts, enzymes or other substances that could affect downstream applications. Examples include cleaning up PCR reactions, digests or other enzymatic treatments and cleaning up genomic or plasmid DNA contaminated with cellular proteins/debris. There are several ways to approach DNA clean-up, here are five of them.

1. Phenol/Chloroform extraction

Phenol chloroform extraction (see Kirby, 1957), normally followed by ethanol precipitation, is the traditional method to remove protein from a DNA sample. In this procedure, the DNA solution is mixed with phenol and chloroform. The water-soluble DNA partitions into the aqueous phase, while the proteins are denatured by the organic solvents and stay in the organic phase. The aqueous phase containing the protein-free DNA can then be collected.

Advantages: A cheap and effective way to remove proteins from DNA solutions
Disadvantages: Slow compared to most modern methods, there is a risk of phenol/chloroform carry-over into the final sample (which could inhibit downstream enzymatic reactions), chloroform and phenol are both hazardous chemicals

Hhhuuummmm Gas cans in the trunk????

[Buckeye]

Nuclear DNA requires a hair follicle (root) to be tested...or tooth, bone, etc.  If there was no hair root, only the shaft, then only mitocondrial testing could be performed.  Like Janet said, the original testing could have been done just to establish death, not paternity.

[KarmaRoundUp]

I tried to find it on my own but failed 
Can I get the stalker cam link again, please?

http://media.myfoxorlando.com/live/mobilecam.html

Is that Lee's car in the driveway?If it is....looks like he took all of the find Caylee writing off of it.Unless I'm not seeing it.I never got an answer to this but does Cindy have a car?

I think it is Jose's car

Ahh,thanks Kristin.

[flamom]

Somehow, I cant picture this family having chloroform at the house of horrors ready to clean up DNA.. They are not a very sophisticated bunch..Whatever they may have done right was just dumb luck IMO

[Brandi]

I can't seem to find any document stating the date LE picked up the Pontiac and took it to their lab.

Can anyone help me on this?

Brandi,I think it was the 17th of June....I could be wrong though.

Thanks, Karma ... I am still searching, though. If anyone knows for sure, please let me know.

[Kristin]

I tried to find it on my own but failed 
Can I get the stalker cam link again, please?

http://media.myfoxorlando.com/live/mobilecam.html

Is that Lee's car in the driveway?If it is....looks like he took all of the find Caylee writing off of it.Unless I'm not seeing it.I never got an answer to this but does Cindy have a car?

I think it is Jose's car

Ahh,thanks Kristin.

Yeah guess they are all getting ready to gatheraround and watch their million dollar show

[anothermonkey]

Casey might not be sophisticated, and neither is Cindy... but George was a detective for how long? Don't you think he has a clue about DNA and how to get rid of "trace evidence"?

[Buckeye]

over on Topix:
http://www.topix.net/forum/county/orange-fl/T4UHQV6DMIUFOA9C5/p1450

Duh in Orlando

Sorry about the brief report earlier. I crashed. This is just a kind of picture of what goes on at TES.

The people who came with horses are assigned to a special group. They are sent out to areas that are pretty wide open. There were 6 private airboats, they were sent out to local lakes, not ponds.

The 150 or so walkers are divided up into groups of 10 with one leader who can read maps, has a gps and a cell phone. One or more groups are given a map of an area to search. The area I went to had 3 groups. We were sent to a rural community about 13 miles from HQ. This community has several large tracks of land around it. Some of those tracks are known for kids partying at night. The one we went to had a main trail. The rest of the area was scrub pines, palmettos and vines. We had two dog teams with us. This gets a little rough, so don't read the rest. We were told we were not looking for the little girl on the T shirts. We were looking for small bones that may have been spread out by animals. Walking thru palmetos is an interesting experience. It turned out that the locals in this area must do a lot of poaching here and dumping of carcassas. We found a lot of bones. It rained most of the day and about 30% of the area was under water. The other problem is this time of year the pines shead there 6 inch needles, which has been made even worse by the recent storms. There is a thick layer of them on the ground. One poor lady found a garbage bag of bones. They turned out to be pig ribs. It rained all day but was about 85 degrees. But strangely I felt cold all day. I think maybe only hunters may know the feeling in a way, that during the season, you look around and at a hundred yards every tree branch looks like antlers. Here it was every white leaf, every white moss stick on the ground looks like a bone. All I can say is there were a lot of very nice folks on a treasure hunt that no one wants to win. But, the more people that are out there, the greater chance of finding something. Before today, I wanted to go out. Now I am compelled. If my legs are working tomorrow.....lol...we will give it a shot. Otherwise, Sunday for sure.

If you guys have any questions let me know.

And let me say again. Tim Miller is the real deal. Probably the only real deal we will meet in this story.

God bless the searchers.

[Kristin]

Somehow, I cant picture this family having chloroform at the house of horrors ready to clean up DNA.. They are not a very sophisticated bunch..Whatever they may have done right was just dumb luck IMO

She could have stolen it gotten it from a friend  person who does the chloroform party?

[TURBOTHINK]

Janet, you're absolutely correct.  They should have qualified the DNA test results.  Unfortunately, I think that there were probably ulterior motives behind releasing the information.  The public was given only limited information, but enough to indicate that there was a likelihood of incest.  Unfortunately, I also think that this was intentional.  I wonder if it was done to create stress with the Anthonys in hopes that one of them would break.

I think they have the tests all back, and I don't think they lead the public to believe there was incest if there wasn't. I have always questioned WHY the big secret and the flat out LIES about the baby's father. I don't think Cindy KNOWS, but I am betting on incest. It is much more common than people realize and Casey is oversexed for a reason. There is also a reason Caylee was described as "being flirty" with men. Those are not normal behaviors in a home with normal father/child/grandchild relationships.